Membrane Vesicle - A Universal Mechanism Functional Transport in the Digestive System

A new mechanism for membrane iron transport in Pseudomonas aeruginosa.

Various biochemical and biophysical studies have demonstrated the existence of a novel iron-uptake mechanism in Pseudomonas aeruginosa, different from that generally described for ferrichrome and ferric-enterobactin in Escherichia coli. This new iron-uptake mechanism involves all the proteins generally reported to be involved in the uptake of ferric-siderophore complexes in Gram-negative bacter...

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane.

A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on s...

Vesicle transport in chloroplasts

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis

Biological membranes in eukaryotes contain a large variety of proteins and lipids often distributed in domains in plasma membrane and endomembranes. Molecular mechanisms responsible for the transport and the organization of these membrane domains along the secretory pathway still remain elusive. Here we show that vesicular SNARE TI-VAMP/VAMP7 plays a major role in membrane domains composition a...

Phylogenetic studies on the synaptic vesicle glutamate transport system.

The ATP-dependent uptake of glutamate into synaptic vesicles isolated form mammalian brains is well characterized. Glutamate uptake requires an electrochemical proton gradient, is specific for glutamate over other amino acids, and is stimulated by chloride. To determine whether these characteristics are fundamental to the vesicular uptake system, vesicles were isolated from the brain and centra...